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Therefore, developing drug delivery carriers capable of sustained, controlled release, such as biodegradable microspheres, thermosensitive or pH-sensitive hydrogels, and various nanoparticles, is crucial for achieving long-term joint protection (Yu et al., 2024

The F2 progeny was subsequently screened for the desired edit by PCR amplification using the adh-5 forward primer (5-CGATCCAAGTGGCTCCACCGAA-3) and the adh-5 reverse primer (5-TTCCACATCCCAAAAGCGAAACC-3)